FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495373
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC77220.5163928999654267No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAG73240.4897774668928756No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATT64830.43353731811394214No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTG56650.37883524712563355No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46860.31336663160295125No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44270.29604653822156746No Hit
CACTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGCAACACGGTCTCCCTGAAAATCT41810.2795957931566238No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC39720.26561934714616353No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG39470.26394752346070177No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38870.2599351466155936No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG38370.25659149924467006No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTT35890.24000700828488947No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC34920.23352033238529785No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA33030.220881345323207No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32990.2206138535335331No Hit
GATTATTACTGCTGCTCACATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT30850.2063030427859805No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30770.20576805920663271No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30730.20550056741695885No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGGAGA30510.2040293625737525No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC30040.20088633404508444No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29830.19948200214929654No Hit
GTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATG28480.19045415424780307No Hit
ACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC28240.18884920350975976No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAAT28010.18731112571913497No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27420.18336562182144522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27070.18102506866179874No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26950.18022259329277712No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26210.17527399518381032No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25730.17206409370772374No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG25630.17139536423353907No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25150.1681854627574525No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24870.16631302022973532No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC24390.16310311875364877No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC23900.15982634433014373No Hit
CTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGC23580.1576864100127527No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22920.15327279548313363No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22890.15307217664087822No Hit
TTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTCTCCTGGTACCAGCAACATCCAGGCAAAGC22670.1516009717976719No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22540.15073162348123176No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22500.1504641316915579No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22140.14805670558449296No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTAC21930.1466523736887051No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCA21780.14564927947742803No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21740.14538178768775414No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21480.14364309105487394No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21460.143509345160037No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20890.1396975871571842No Hit
CATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC20700.13842700115623327No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20520.1372232881027008No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20070.13421400546886963No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC19700.13173970641438623No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19340.1293322803073213No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC19120.127861075464115No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19040.1273260918847672No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT18870.1261892517786532No Hit
CTCTTAGTGTCCTCATAAATTATGAGTCGGGGGGCTTTGCCTGGATGTTG18350.12271185851289276No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT18210.12177563724903419No Hit
GTTCTCTGGTTCCAAGTCTGGCAACACGGTCTCCCTGAAAATCTCTGGGC18090.12097316188001254No Hit
CCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCG17730.11856573577294761No Hit
CCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTC17710.11843198987811067No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17690.11829824398327375No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17510.11709453092974129No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC17460.11676016619264892No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17210.11508834250718716No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAG16330.1092035231343618No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCA16080.10753169944890005No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15970.10679609702729687No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15950.10666235113245992No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15660.10472303565732428No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15640.10458928976248737No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15510.10371994144604724No Hit
GTACCAGCAACATCCAGGCAAAGCCCCCCGACTCATAATTTATGAGGACA15510.10371994144604724No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15440.10325183081411796No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG15280.10218186365542244No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAG15260.10204811776058549No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGG15190.1015800071286562No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15070.10077753175963455No Hit
GTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC15040.10057691291737914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.046.4464231
GTATCAA39700.041.4095761
TATCAAC44350.036.8905452
ATCAACG47550.034.7507253
TCAACGC47500.034.7126664
CAACGCA48600.034.0683485
AACGCAG49900.033.3873146
ACGCAGA53250.031.2868927
TACTCGC553.327446E-431.2501920
AGAGTAC53450.031.04128511
CGCAGAG54000.030.9159628
CAGAGTA54450.030.53428510
GCAGAGT55550.029.9914849
GTGGTAT5250.029.4792611
CCTATAC953.3266224E-728.9291523
ACTGGTG48400.028.05259570
TGGTATC5550.026.6303712
AGTACGG35300.026.1768813
GAGTACG35450.026.16301712
AGTACTT28450.025.47654313
Produced by FastQC (version 0.11.5)