FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020242
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54120.267888698482657No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT49260.24383217456126544No Hit
GTAATAAACAGCCGTGTCGTCGACTCGCAGGCTGTCCATTTGGAGATACA42900.21235079757771594No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42080.2082918779037363No Hit
GAGTTTACACTCAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAAACTTTACAACCT41030.20309448076022574No Hit
GTTTATTACTGTGCGAGAGAGAGGACTCGAAGGTCACCACTACCAACTGGGATTGACTACTGGGGCCGGGGAACC39020.19314517765693417No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38220.1891852560237833No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35510.17577102149148469No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG35260.17453354598112503No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC33910.16785117822518292No Hit
GTAATGAATACACCTATTTCCATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGTTT33500.1658217183881931No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32280.159782837897638No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32160.15918884965266536No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC30090.14894255242688748No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29900.14800207103901414No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGCTCTGGCACAGAGTTTACACTCAA28640.1417651944668015No Hit
GTGTAAACTCTGTGCCAGAGCCACTGCCACTGAACCTGTCAGGGACCCCG28530.14122070524224326No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28480.14097321014017133No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28460.14087421209934256No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28150.1393397424664966No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27940.1383002630377945No Hit
GGTCTAGTCAGAGCCTCCTGCATGGTAATGAATACACCTATTTCCATTGGTACCTGCAGAAGCCAGGGCAGTCTC27870.13795376989489377No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27700.13711228654784924No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT26600.13166739430226676No Hit
GGCCAAAAGTGTACGGAGGTTGTAAAGTTTGCATGCAGTAATAAATCCCA26550.1314198992001948No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAACCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA25790.1276579736487015No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC25620.12681649030165693No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGTTTCTAATCGGGCC24730.12241107748477659No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA24340.12048061568861552No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG23930.1184511558516257No Hit
CCCCGGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG23800.11780766858623867No Hit
GTTCAGTGGCAGTGGCTCTGGCACAGAGTTTACACTCAAAATCAGCAGAG23620.11691668621877972No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC22820.11295676458562885No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22330.11053131258532395No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22290.1103333165036664No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCT22040.10909584099330674No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21980.10879884687082042No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21160.10473992719684078No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20860.10325495658440918No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20710.1025124712781934No Hit
CTCCTGTGCAGCGTCTGGATTCACCCTCAGCTTCTATGCCATGCACTGGGTCCGCCAGTCTCCAGGCAAGGGG20300.10048301144120358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.044.7858851
GTGGTAT4050.039.9346281
GTATCAA31300.039.2491871
CCTTTGT17700.037.62588570
TATCAAC35750.034.2665182
TGGTATC4750.034.048682
ATCAACG38050.032.5569573
TCAACGC38200.032.4307254
CAACGCA38400.032.1729975
AACGCAG39550.031.23756
ACGCAGA41900.029.3168957
TGCGAAA605.511113E-428.67149212
AGAGTAC43450.028.19048111
CGCAGAG43650.028.1415318
TCTGGAG20550.027.6418670
CAGAGTA44550.027.4957810
ACTAGCG1705.456968E-1226.33051327
TAACGGC1901.8189894E-1225.44357336
TGTGCGA15000.025.23278410
TACACCC15900.023.808055
Produced by FastQC (version 0.11.5)