FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837424
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48010.26128971864958767No Hit
GTAATAGACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA41540.22607737789426935No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37140.20213080921986432No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT33680.18330009839862763No Hit
GTCTTCAGGCTCTAGGCTACTGATGGTGAGAGTGAAGTCTGTCCCAGCCC32370.1761705518160207No Hit
GTCTATTACTGTGCCAGAGACGCAACCCTGGTACCAGCGCCTATAAGTTA28740.15641463265963654No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28740.15641463265963654No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28470.15494518412734348No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28250.15374785569362326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28090.15287707137819034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26550.14449577234214855No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25530.13894452233126378No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24960.13584235320753402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23500.1278964463292087No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22850.12435888504776253No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22700.12354252475204416No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22600.1229982845548986No Hit
GTTTATTACTGTCAGCAGCGTACCATCTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA22460.1222363482788948No Hit
CTCCTGCAGGACCAGTCAGCCTGTTAACAGTCACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT21700.11810012278058847No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21690.11804569876087392No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21420.11657625022858087No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG21150.11510680169628784No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20190.10988209580369039No Hit
CCTGAAGACTGTGCAGTTTATTACTGTCAGCAGCGTACCATCTGGCCTCT20180.10982767178397583No Hit
GTAATAAACTGCACAGTCTTCAGGCTCTAGGCTACTGATGGTGAGAGTGAAGTCTGTCCCAGCCCCACTACCA19730.10737859089682077No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19640.10688877471938976No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19180.10438526981252014No Hit
CTGCAGGACCAGTCAGCCTGTTAACAGTCACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCGT18990.10335121343794357No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG18780.10220830902393788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.040.31921
GTATCAA35400.038.2832261
CTACGCT1550.035.53228428
TATCAAC39800.033.9644852
ATCAACG42000.032.2672923
TCAACGC42350.032.0835844
CAACGCA43600.031.4801945
AACGCAG43850.031.3024226
TCTGGAG20900.029.35848270
CGCGTTG12750.028.60651461
TTCGCGT12500.028.55096259
CGCAGAG48700.028.321698
GCGTTGT13150.028.30037362
AGAGTAC48650.028.0664711
TCGCGTT12900.027.96786760
ACGCAGA50550.027.1491017
GTCGGCT14850.026.86799619
GCAGAGT51500.026.7150829
CGTTGTC14250.026.63887463
TGTTAGA6550.026.47427670
Produced by FastQC (version 0.11.5)