FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837424
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48690.2649905519901775No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34350.18694650771950294No Hit
GTAATAGACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA33900.1844974268323479No Hit
GTCTTCAGGCTCTAGGCTACTGATGGTGAGAGTGAAGTCTGTCCCAGCCC32260.17557188759916056No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30500.16599326012939855No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT29840.1624012748282378No Hit
GTCTATTACTGTGCCAGAGACGCAACCCTGGTACCAGCGCCTATAAGTTA29470.1603875860987992No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28440.15478191206819983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27880.15173416696418465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26110.14210111547470805No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25870.1407949390015587No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25860.14074051498184414No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25310.1377471938975435No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24640.1341007845766682No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG24000.13061764731493655No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21760.11842666689887582No Hit
CTCCTGCAGGACCAGTCAGCCTGTTAACAGTCACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT21140.1150523776765733No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21120.11494352963714417No Hit
GTTTATTACTGTCAGCAGCGTACCATCTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20810.11325638502599292No Hit
CTGCAGGACCAGTCAGCCTGTTAACAGTCACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCGT20480.11146039237541253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19770.107596286975679No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19700.1072153188376771No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18680.1016640688267923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.040.421081
GTATCAA35550.039.4570851
TATCAAC40800.034.358352
TCAACGC42500.032.794644
ATCAACG42650.032.7651443
CAACGCA43000.032.17385
AACGCAG44400.031.3139486
TTCGCGT12400.030.50068759
TCTGGAG18800.030.09101170
CGCGTTG12750.030.00888361
TCGCGTT12650.029.66141960
GCGTTGT13000.028.92636362
AGAGTAC48550.028.63726411
CGCAGAG48800.028.4905598
CGTTGTC13450.027.74209663
ACGCAGA50800.027.3013067
GCAGAGT51700.026.7596429
CTTCGCG14650.026.5113258
GTTGTCT14400.026.45024164
CAGAGTA52750.026.29206510
Produced by FastQC (version 0.11.5)