FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196735
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG704.2564352E-1044.53835740
GTATCAA43150.042.1087271
GGTATCA17150.041.3013841
TATCAAC48450.037.5024032
GTGGTAT4800.036.3569371
TCAACGC52050.035.042994
ATCAACG52000.035.009413
CAACGCA52200.034.9437525
AACGCAG53300.034.352446
CTTATAC3700.032.368661
ACGCAGA58000.031.5687037
CGCAGAG58300.031.3475578
CGTATGC1251.1641532E-1030.53051841
AGAGTAC59250.030.4970911
ACCGATC3200.029.9898827
CAGAGTA60300.029.96729910
TATACAC4250.029.7912923
GCAGAGT62550.029.2723439
CTCGTAT953.118039E-729.14296739
AGTATCG1404.802132E-1027.04400434
Produced by FastQC (version 0.11.5)