FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779331
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT119920.4314707388216804No Hit
CTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT102100.3673545900074514No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG89260.32115642217497664No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC87160.3136006470621887No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGCTGTAAAAAACACT82210.29579060572490284No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC76210.27420267683122307No Hit
ATCGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA74940.26963323188206084No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG73630.26491986740694073No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT73430.26420026977715144No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG71820.2584075088573473No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC70610.2540539431971219No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC69590.25038399528519634No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC68250.24556269116560786No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTA66700.23998580953474058No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63510.2285082273396008No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAA62220.22386682262745963No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC60280.21688672561850317No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC59950.21569938952935075No Hit
GTCTCTGGTGGTTCCATTAGTAGTCACTTCTGGAGCTGGATCCGGCAGCC59630.21454803332168784No Hit
GTAATAAAGGGCCGTGTCCGCAGCGGTCACGGAGGTCAGCTTCAGGGAGA58110.20907909133528899No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT56920.20479748543804246No Hit
GTTTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA56500.20328633041548486No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC54880.19745758961419135No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT51990.18705940386373557No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG51700.18601598730054103No Hit
GAGTACGATAATTTTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT51050.1836772950037257No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG50250.18079890448456842No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAA48590.17482624415731698No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC48290.173746847712633No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC47630.1713721755343282No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTT47400.1705446382600705No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47180.16975308086730226No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA46830.1684937850151709No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC46790.16834986548921307No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACT46470.16719850928155014No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG46200.16622705248133454No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT45680.1643560986438823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45280.16291690338430365No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAACAATAAGAACTT45090.16223328563600378No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG44970.16180152705813017No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43480.15644052471619968No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC43300.1557928868493893No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGCTG42680.15356213419704237No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA42670.15352615431555292No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42600.15327429514512664No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG42070.15136736142618493No Hit
CACCTGCACTGTCTCTGGTGGTTCCATTAGTAGTCACTTCTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA41920.15082766320384294No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACTCCGC41620.14974826675915895No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA40710.1464740975436175No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40080.14420736500978115No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39990.14388354607637593No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG38990.1402855579274293No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG38780.1395299804161505No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT38540.1386664632604033No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG38420.1382347046825297No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT38270.13769500646018773No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA37510.13496053546698827No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC37130.13359329997038857No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36280.1305350100437839No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT35930.1292757141916526No Hit
GTCCTGGACCTCCTGTGCAAGAAGATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC35540.1278724988135634No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT35190.12661320296143208No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA35030.12603752485760064No Hit
GTTTTTTACAGCTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA35020.12600154497611116No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT34440.12391471184972211No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG34290.12337501362738011No Hit
CTTCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATC33910.1220077781307804No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA33430.120280743819286No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33420.12024476393779654No Hit
GTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA33010.11876958879672843No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT32900.1183738101003443No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG32880.11830185033736536No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG32200.11585521839608164No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA32150.11567531898863431No Hit
CTCCAGAGTCGAGTCACCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCCGTGACCG31760.11427210361054513No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA31660.11391230479565045No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC31330.11272496870649808No Hit
CTTTATTACTGTGCGAGAATGCCTGACGTAAAAAATGATGCCTTTGATCT31220.11232919001011395No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC31160.11211331072117714No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA30930.11128577344691944No Hit
TTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTTCGGCGGAGGGA30930.11128577344691944No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT30760.1106741154615985No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC30480.10966667877989345No Hit
ACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT30360.10923492020201983No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC30200.10865924209818839No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA30050.1081195438758464No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC29670.10675230837924665No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA29370.10567291193456267No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT29260.10527713323817854No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC29220.10513321371222067No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT28880.10390989774157881No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA28810.10365803857115255No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC28790.10358607880817361No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA28620.10297442082285269No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC28400.10218286343008443No Hit
ATGCTGGGAGCATCGTCTACAACCCCGCCCTCCAGAGTCGAGTCACCATA28050.10092356757795311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.046.2635651
GTGGTAT4400.043.751781
CTAGCGC2500.042.66127428
GTATCAA40650.041.6068571
TCGTATG1500.041.48379540
CGTATGC1450.040.55729341
ACTAGCG2650.040.2399627
TAGCGCT2600.039.7079429
ATACCAT350.001371778939.2894366
TGGTATC4850.038.9835432
CTTATAC3000.037.8140371
TATCAAC46600.036.3681642
AGACTAG3200.035.4634225
ATATACC601.3766539E-534.37644
GATCGTA400.002643074634.3763963
ATCTCCG3400.034.37578210
ATCAACG49650.033.995593
TATACAC3550.033.8922233
CGAGACT3350.033.8694123
ACGAGAC3400.033.3683322
Produced by FastQC (version 0.11.5)