FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834084
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCA59290.7108396756202012No Hit
CTCCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC57170.68542257134773No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55660.6673188791536584No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC39930.4787287611319723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38110.4569084168980582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37540.45007457282479946No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37220.44623802878367164No Hit
GCATAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCTCCGGGGTCCCTGACAGGTT34890.4183031924842102No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34200.41003064439552855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33770.40487528834026304No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32050.3842538641192014No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCC31730.38041732007807366No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29320.35152334776833033No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29300.3512835637657598No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT29020.34792658772977303No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA27660.3316212755549801No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26920.32274926745987215No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGACACAACTATTTGGATTGGTACCTGCATAAGCCAGGGCAGTCTC26700.3201116434315968No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26180.3138772593647642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26170.313757367363479No Hit
GGCTTATGCAGGTACCAATCCAAATAGTTGTGTCCATTAGTATGCAGGAG24650.2955337831681221No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24430.29289615913984685No Hit
GTCTCCACTCTCCCTGCCCGTCATCCCTGGGGAGCCGGCCTCCATCTCCT24290.2912176711218534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23480.2815064190177488No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG23200.27814944298176203No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTATGCAGGTA23100.2769505229689096No Hit
CTACAAACTCCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACG22010.2638822948288182No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCC21790.26124467080054287No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21390.2564489907491332No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21390.2564489907491332No Hit
TCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG20980.25153341869643825No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20770.24901568666944818No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC20490.2456587106334614No Hit
GCCCTGGCTTATGCAGGTACCAATCCAAATAGTTGTGTCCATTAGTATGC20160.2417022745910484No Hit
GTACCTGCATAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCTCCGGGGTCCCTGA19860.2381055145524911No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19750.23678670253835343No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19380.23235069849079948No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT19280.23115177847794705No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCATCC19210.23031253446895036No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19200.23019264246766513No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18920.22683566643167835No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18810.22551685441754066No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA18730.22455771840725874No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18570.2226394463866949No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18270.2190426863481376No Hit
CTAATGGACACAACTATTTGGATTGGTACCTGCATAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT18130.2173641983301442No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT18030.2161652783172918No Hit
GCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCAGG18030.2161652783172918No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC17090.204895430196479No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16810.2015384541604922No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16690.2000997501450693No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16340.19590353010008585No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTGGGACGTTCGGCCAAGGGA16340.19590353010008585No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16270.19506428609108914No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16260.1949443940898039No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16100.19302612206924002No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16000.19182720205638762No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA15990.19170731005510236No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15800.18942936203068275No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA15630.18739119800883364No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCTGGGACGTTCGGCCAAGGGA15500.1858326019921255No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14860.17815951390987No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14800.17744016190215853No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14600.1750423218764537No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT14510.17396329386488651No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG14510.17396329386488651No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC14510.17396329386488651No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14380.17240469784817836No Hit
CAACTATTTGGATTGGTACCTGCATAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGC14230.17060631782889973No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13930.16700955779034246No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCTCCGGGGTCC13900.16664988178648674No Hit
CTCCCTGCCCGTCATCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGTCTA13720.16449182576335236No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCTCCGGGGTCCCT13590.1629332297466442No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13410.16077517372350986No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13170.15789776569266406No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12880.15442089765539202No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC12800.1534617616451101No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12790.15334186964382485No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12320.14770694558341846No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12230.14662791757185126No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12030.14423007754614642No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11980.14363061753972023No Hit
AGTCAGAGCCTCCTGCATACTAATGGACACAACTATTTGGATTGGTACCTGCATAAGCCAGGGCAGTCTCCACAG11780.1412327775140154No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCATCCCTGGGGAGCCGGCCTCCATCTCCTGCAGGT11750.14087310151015964No Hit
GTGGAGACTGCCCTGGCTTATGCAGGTACCAATCCAAATAGTTGTGTCCA11750.14087310151015964No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11680.14003385750116296No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11660.13979407349859246No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11430.1370365574690319No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11380.13643709746260568No Hit
ATGCAGGTACCAATCCAAATAGTTGTGTCCATTAGTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGC11270.13511828544846802No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11220.1345188254420418No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11080.1328403374240484No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG11060.13260055342147792No Hit
GCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCT11040.13236076941890745No Hit
CCTGCATACTAATGGACACAACTATTTGGATTGGTACCTGCATAAGCCAG10900.13068228140091404No Hit
GGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATT10860.13020271339577308No Hit
CCACTGAACCTGTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGG10850.13008282139448785No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10700.1282844413752092No Hit
ATTTGGGTTCTACTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT10570.12672584535850107No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10520.12612638535207485No Hit
ATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGA10460.1254070333443634No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10360.12420811333151097No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10200.1222898413109471No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10040.12037156929038322No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10040.12037156929038322No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9990.11977210928395701No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9810.11761405326082267No Hit
GGTTTATTACTGCATGCAAGCTCTACAAACTCCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA9780.11725437725696693No Hit
CTCCTGCATACTAATGGACACAACTATTTGGATTGGTACCTGCATAAGCCAGGGCAGTCTCCACAGCTCCTGAT9750.1168947012531112No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA9700.11629524124668499No Hit
GCTGTGGAGACTGCCCTGGCTTATGCAGGTACCAATCCAAATAGTTGTGTCCATTAGTATGCAGGAGGCTCTGA9700.11629524124668499No Hit
GCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTC9590.11497642923254732No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9570.11473664522997683No Hit
CCTTGGTCCCTTGGCCGAACGTCCCAGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT9430.11305815721198345No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9320.11173934519784579No Hit
AGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT9210.1104205331837081No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9200.11030064118242287No Hit
CCACTCTCCCTGCCCGTCATCCCTGGGGAGCCGGCCTCCATCTCCTGCAG9150.10970118117599667No Hit
GGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC9140.10958128917471141No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9090.10898182916828521No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCATCCCTGGGGAGCCGG8990.10778290915543279No Hit
CAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTG8970.1075431251528623No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8970.1075431251528623No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGC8930.10706355714772134No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8900.1067038811438656No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA8820.10574474513358367No Hit
GCATACTAATGGACACAACTATTTGGATTGGTACCTGCATAAGCCAGGGC8710.104425933119446No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT8700.10430604111816076No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8360.10022971307446253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGG100.0076694004141.23570
ATTCTCG200.00738779551.6976937
GCTTGCG200.007533374751.44317226
GTTCGTA200.007542319651.4277341
GTGGTAT1350.050.7928241
ATAAACG451.9562594E-645.6669463
TAAACGC451.9586842E-645.6587334
GTATCAA14750.043.699051
GGTATCA5350.039.732331
TGGTATC1650.039.4561962
TATCAAC17900.035.9873922
CGTATAT708.185252E-735.2716962
TCGTATA708.2646875E-735.22818861
CTCGTAT708.356874E-735.17828860
ATCAACG18450.035.085583
TCAACGC18550.034.8901634
AACGCAG19250.034.866676
GTGCTTA400.002676671634.2851561
ACTTATG701.0262393E-634.26664721
AGGTACG400.002684558734.2645872
Produced by FastQC (version 0.11.5)