FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996905
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17220.17273461362918233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.038.3657261
GGTATCA11100.031.826711
TATCAAC30550.031.5258872
CAACGCA31000.031.1537915
AACGCAG31150.031.0037776
TCAACGC31150.030.8938354
ATCAACG31700.030.471973
ACGCAGA32900.029.4587337
AGAGTAC32900.028.8327211
CGCAGAG34400.028.2723318
CAGAGTA33750.028.2080310
CCGTTAA1353.3287506E-1027.90922516
GTGGTAT5700.027.0779111
GCAGAGT36700.026.687129
GTATAGC951.0993639E-525.2727181
AGTACGG28400.024.47819713
CGGATAG700.00138848424.47324620
GCCGTTA1551.6589183E-924.30437915
GAGTACG28800.024.2571312
TGGTATC6300.023.9378052
Produced by FastQC (version 0.11.5)