FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419598
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36360.25612884774422057No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31160.2194987595079734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30250.21308849406663014No Hit
ATATGATACTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCAT30200.21273628167974312No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25790.18167114915631044No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG25660.18075539695040427No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTG24160.17018902534379451No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24160.17018902534379451No Hit
GTATCATATTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC23210.16349698999294165No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23180.16328566256080945No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22990.1619472554906389No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21760.1532828307732189No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21280.14990159185910376No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20200.14229380430234476No Hit
GTAATAGACGGCCGTGTCCGCAGGTGTCACAGAGTTCAGCCTCAGGGAGA19480.13722194593117207No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGACTCACG18890.13306583976590555No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18790.13236141499213155No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18560.1307412380124514No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGACTCACGACATTAGCAA17860.12581026459603353No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17780.12524672477701435No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17710.12475362743537255No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA17460.1229925655009376No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16890.11897734429042588No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT16720.1177798221750101No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16050.1130601761907244No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA15560.1096084947992319No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14690.10347999926739823No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGACTCACGACATTAGCAACCATTTAAATTGGTATCAGCAGAAA14360.10115539751394409No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC14310.10080318512705709No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14300.1007327426496797No Hit
GATCTACGATGTATCCAATTTGCAAACAGGGGTCCCATCAACGTTCAGTGGAAGTGGATCTGGGACAGATTTTA14280.1005918576949249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27800.035.997651
TACTGAT10000.035.6086970
ATCGCGT400.002600076134.4904137
TATCAAC31750.031.51922
CTACGCT2200.031.19788228
TCAACGC32300.030.8785654
TGTTAGA8850.030.5494170
CAACGCA33050.030.1778435
AACGCAG33300.029.9512836
GTGGTAT4900.028.6763481
TAGACGG7550.027.6916945
TTGGACG7450.026.9367359
CGCAGAG37200.026.626028
ATAGACG8000.026.5624644
TACGCTG2600.026.40658829
AATCGAG1258.9684414E-426.37680670
ATCTCCG2600.026.36287310
AGAGTAC37550.026.28471811
CAGAGTA38250.025.894210
CTTATAC3050.025.8442571
Produced by FastQC (version 0.11.5)