FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419598
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35390.24929592743861292No Hit
ATATGATACTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCAT28940.20386052953019093No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28450.20040884813869844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27410.19308283049144898No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27360.19273061810456202No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25230.17772637042317613No Hit
GTATCATATTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC25190.17744460051366653No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24110.1698368129569075No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23630.1664555740427924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22840.16089061832997792No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTG21980.15483256527552167No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21750.1532123882958415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21660.15257840599944492No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19650.13841946804658783No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGACTCACG19650.13841946804658783No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGACTCACGACATTAGCAA19530.13757415831805905No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGACTCACGACATTAGCAACCATTTAAATTGGTATCAGCAGAAA19160.13496778665509532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19020.13398159197181175No Hit
GTAATAGACGGCCGTGTCCGCAGGTGTCACAGAGTTCAGCCTCAGGGAGA17670.12447185752586296No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16990.11968176906419986No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA16870.1188364593356711No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16760.1180615920845197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16370.11531433546680117No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15960.11242619389432783No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT15820.11143999921104425No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15380.10834053020643873No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA15050.10601592845298459No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15050.10601592845298459No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14550.10249380458411467No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14450.10178937981034067No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14270.10052141521754751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28050.039.026691
TATCAAC33300.032.74192
TCAACGC33700.032.2265474
CAACGCA34100.031.8496445
AACGCAG35500.030.9796416
GTGGTAT5150.030.6516971
AGAGTAC37900.028.92747111
AGGTACG953.3837568E-728.8727022
CGCAGAG38950.028.3235768
ACGCAGA39550.027.8938887
CAGAGTA39500.027.75572410
GCAGAGT40000.027.5800849
TAGGTAC1058.143834E-726.11187622
AGTACGG29750.025.56527313
GAGTACG30250.025.14270412
TGTTAGA8600.023.2909970
TCTAGAC902.2281546E-422.8422583
TAAACGC902.2297497E-422.8398444
ATCAACG49450.022.1031743
GAGTACT15400.022.02413612
Produced by FastQC (version 0.11.5)