FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3622865
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT117870.3253502407624904No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG79710.22001923891726577No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC78650.2170933777548984No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG73370.2025192768706535No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC72840.20105634628946978No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG71100.19625351758897996No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG70580.19481818947159224No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT70140.19360368106457182No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC69450.19169911106265344No Hit
GGTCTAGTCAGAGCCTCCTGCATTTTAATGGGAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC66060.1823418758358371No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA64460.17792548162849015No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC63830.17618652640934732No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT60900.16809900451714319No Hit
GTATATTACTGTGCGAGAAGTCGGGAGGGGAGGCAGTTACTCGTTCCCGT60210.1661944345152248No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT57890.15979066291457175No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT57450.15857615450755133No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG57130.15769287566608195No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC53840.14861166507722479No Hit
GGCCAAAAGTGTACGGAGTTTGTAGAGTTTGCATGCAGTAATAAACTCCA52950.14615504579938804No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT52480.14485773000097987No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT51840.1430911723180411No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT51790.1429531599990615No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51600.14242871318693906No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT51140.14115899985232683No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC51130.1411313973885309No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA50910.14052414318502068No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA50780.14016531115567374No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT47970.13240901882902067No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC47540.1312221128857962No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47150.13014561679775535No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG47080.12995239955118393No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG46860.12934514534767375No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA46720.1289587108545309No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA45920.1267505137508574No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC44740.12349342302293903No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG44580.12305178360220433No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44420.12261014418146965No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA43210.11927024606216352No Hit
CTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA42340.11686883171191861No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC41790.1153506962031431No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC40910.11292167938910228No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40770.11253524489595941No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG40280.1111827241699594No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACT40220.1110171093871839No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA39630.10938856402322472No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38950.10751159648510226No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38690.10679393242640839No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC38190.10541380923661245No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38010.10491696488828593No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT37910.10464094025032673No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA37660.10395087865542878No Hit
GTTTGTAGAGTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT37600.10378526387265327No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC37320.10301239488636756No Hit
GTTTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA37020.10218432097249001No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36850.10171507908795939No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA36810.10160466923277572No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAACTCTACAAAC36660.10119063227583694No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36260.10008653372400021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.044.4611551
GTATCAA61100.040.9728431
GTGGTAT6750.037.1899071
TATCAAC70600.035.4107972
TCGTATG2300.034.5985340
TCAACGC75750.033.0959474
CAACGCA77900.032.139265
AACGCAG79750.031.4368346
CTCGTAT2450.029.63736339
AGAGTAC85450.029.21383511
CGCAGAG86100.029.0352298
CGTATGC3050.028.37797541
TGGTATC8750.028.296352
CAGAGTA89000.028.16602110
ATCAACG91500.027.360013
ACCCAGG33150.026.23883870
AATATAC20600.026.0413423
ATACGGC20500.025.835026
ACGAGAC3500.025.54672422
TAATATA20900.025.338472
Produced by FastQC (version 0.11.5)