FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384014
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAT350.00141914539.0104751
TCGTATG557.160146E-637.80356240
CTCGTAT557.2647817E-637.72374339
GTATCAA10350.034.6288641
GGTATCA4950.034.478961
CTTATAC807.768176E-834.1341671
TGCACTA400.00273339634.1341675
CGTATGC802.637009E-630.35773841
TATCAAC12050.029.4601962
AACGCAG12250.028.9792126
CAACGCA12850.027.6260975
ATCAACG12900.027.5190183
TCAACGC12900.027.5190184
TATAGGG500.008141234527.3073332
ACGCAGA13100.027.0882957
TTATACA1156.2305844E-826.7136962
TAACCGT808.367811E-526.38067253
TACGTGG659.000667E-426.35661531
CGCAGAG13500.026.2856798
AGAGTAC13050.026.14623311
Produced by FastQC (version 0.11.5)