FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513066
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8090.15767951881434358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.040.323391
TATCAAC16450.033.5692982
GGTATCA5800.033.1292651
ATCAACG16850.032.7468453
TCAACGC16900.032.6467824
AACGCAG17350.032.3925826
CAACGCA17400.031.7086525
GTGGTAT2300.031.3287621
ACGCAGA18600.030.215667
CGCAGAG19450.028.895188
AGAGTAC19600.028.32436211
CAGAGTA19750.028.28275510
GCAGAGT21000.026.9256279
AGTACGG16300.024.3877413
GAGTACG16500.024.0921312
CCGACAC750.001852183523.28856351
TACACTC750.002070859822.8459875
CGTCCTA952.6398018E-422.27305660
TAATACC953.2047057E-421.64356617
AGTACTT7300.021.59415213
Produced by FastQC (version 0.11.5)