FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983568
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23740.11968331814185347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76850.040.6692431
GGTATCA31500.039.3402561
ACGCGAA350.001400795439.1219211
ACGTCGA451.0491156E-438.0342412
TATCAAC85450.036.7373122
ATCAACG89150.035.13583
TCAACGC89200.035.116994
CAACGCA91350.034.36635
AACGCAG93750.033.4508486
TCGTATG3550.033.1741440
CGTATGC4300.030.65071941
ACGCAGA103500.030.3304677
AGAGTAC106700.029.64392911
CGCAGAG106150.029.6055268
TATGCCG4250.029.43929343
CAGAGTA108750.029.08585510
GCAGAGT111600.028.3430739
ACGAGAC5200.028.3119622
AGTACGG52600.027.33190213
GAGTACG52750.027.2548712
Produced by FastQC (version 0.11.5)