FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983568
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44560.22464568898066514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79200.041.0026861
GGTATCA31250.039.379151
TATCAAC85750.037.7623752
ATCAACG91750.035.1962473
TCAACGC92100.035.0227784
CAACGCA94500.034.204695
AACGCAG96750.033.5856256
TGGTATC13600.030.8923742
ACGCAGA106900.030.396727
CGCAGAG108900.029.838478
AGAGTAC109550.029.63027211
GTGGTAT14650.029.3999651
CAGAGTA111050.029.29151510
GCAGAGT113700.028.5787969
GAGTACG54050.025.82825912
AGTACGG54250.025.79595413
AGTACTT73500.024.98402813
GAGTACT74800.024.68670712
CGACCGG2301.4893703E-724.42281270
TACGGGG31350.023.9544115
Produced by FastQC (version 0.11.5)