FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90034
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1600.17771064264611147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTGT204.7815824E-4102.9285370
GTGCGGG400.00747522851.46426470
TATAGGA200.007532553751.4070852
GTGGTAT507.217932E-847.9799461
GTCCACA306.489504E-445.6951871
TGGTATC651.1859811E-842.1801722
GTATCAA3100.040.904561
GTGTAGA451.0339262E-438.0793231
TATCAAC3350.036.8289532
AACGCAG3600.034.271396
TCAACGC3600.033.3194054
CAACGCA3650.032.8629765
ATCAACG3650.032.8629763
GGTATCA1154.9112714E-1132.781331
ACGCAGA3950.031.2346847
CGCAGAG4050.029.617258
AGAGTAC4150.028.07776511
GCAGAGT4300.027.8953179
CAGAGTA4400.027.26133310
TACTTTT1401.45100785E-824.47956515
Produced by FastQC (version 0.11.5)