FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310920
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15240.11625423366795838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34000.039.0069851
GGTATCA14750.035.173771
TATCAAC38850.034.0307582
ATCAACG39800.032.9457863
GTGGTAT6550.032.5225371
TCAACGC40150.032.310454
CAACGCA41050.031.6856655
AACGCAG43500.029.901076
TGGTATC7250.028.8930722
ACGCAGA46250.028.1231677
CCGGTAA600.00709046128.09617469
CAGAGTA47600.027.39765510
CGCAGAG48200.027.1278088
AGAGTAC49150.026.67329211
GCAGAGT52050.025.0553029
GTATATA2800.023.3146861
AGTACGG45200.022.7782113
GAGTACG45950.022.48111212
TATCCCG800.002989414821.4494825
GTATAAG3300.020.8233241
Produced by FastQC (version 0.11.5)