FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488935
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8970.18345996911654924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT150.00216809170.4516360
GTATCAA13900.039.7275161
GGTATCA6300.034.843341
TCAACGC15800.033.6098184
GTGGTAT2750.032.4280661
CAACGCA16800.032.4249575
TATCAAC16600.032.4161032
AACGCAG17150.031.9629886
ATCAACG16850.031.5218853
ACGCAGA17550.031.0392747
AGAGTAC17400.030.71615811
CGCAGAG17900.030.049568
CAGAGTA17950.029.58412710
GCAGAGT19600.027.4432229
GCGTTAT809.937931E-525.72418626
AGTACGG16900.025.3404813
GAACTAG951.0978138E-525.2728841
TGGTATC3300.024.92682
GAGTACG17450.024.73811512
GTCCAAT851.4999665E-424.2111
Produced by FastQC (version 0.11.5)