FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679350
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30410.18108196623693693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55550.034.199931
TATCAAC66000.028.66042
TCAACGC66100.028.644614
AACGCAG65650.028.6338376
CAACGCA66550.028.5019975
ATCAACG66600.028.3316973
GGTATCA27800.028.2906021
ACGCAGA69400.027.0376387
AGAGTAC69600.026.71494911
CAGAGTA70700.026.3481610
CGCAGAG73100.025.9016238
GTGGTAT15100.025.2531811
GCAGAGT74900.025.2337689
TGGTATC15100.024.7837542
AGTACGG54050.022.45167413
GAGTACG55000.022.24928512
GAGTACT32150.019.87710412
TACGGGC14300.019.25590715
CGAACTA2506.002665E-1119.09576429
GTACGGG65200.018.56002814
Produced by FastQC (version 0.11.5)