FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404366
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCT28590.11890868528335535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41400.038.3937151
GGTATCA19450.037.4043771
TATCAAC47750.033.1435362
ATCAACG49350.031.929243
TCAACGC50150.031.626164
CAACGCA50500.031.3386945
AACGCAG52550.030.1817686
ACGCAGA58100.027.235917
AGAGTAC58000.027.1045511
CGCAGAG59900.026.4750238
CAGAGTA59650.026.47039410
GTGGTAT7550.025.1174351
GCAGAGT63600.024.8806049
TAATGCG902.1362207E-422.9857821
AGTACGG50300.022.5493113
TTCGGGG13150.022.45079270
GAGTACG51300.022.0425512
TAATATA11400.020.5666542
GTAATAT11250.020.5343911
GTATGTA12150.020.432231
Produced by FastQC (version 0.11.5)