FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404366
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAGTCCGGGGTCCCT25060.10422706027285364No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24200.10065023378304301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.039.1030461
GTATCAA44700.035.101751
GTGGTAT8550.033.879581
TATCAAC49200.031.7913362
TCAACGC52350.029.8585054
ATCAACG52600.029.7184473
CAACGCA53600.029.4191155
AACGCAG55200.028.6917256
TGGTATC9800.027.7727582
ACGCAGA61700.025.66917
AGAGTAC61050.025.65988211
CGCAGAG62000.025.5443618
CAGAGTA62200.025.24081610
GCAGAGT66600.023.8317329
AGTACGG55400.021.00519813
GAGTACG56350.020.77370312
GTATACG1356.8548925E-620.4353051
CGATTCA12200.018.56433954
TTCGGGG11850.017.75088570
CTACCCG12450.017.71335828
Produced by FastQC (version 0.11.5)