FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179698
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.10918943816987492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75750.043.183711
GGTATCA26900.040.6623651
TATCAAC83200.039.2756842
ATCAACG89950.036.4046133
TCAACGC90200.036.2665334
CAACGCA91600.035.7496765
AACGCAG94400.034.6900986
ACGCAGA102900.031.7853837
CGCAGAG103300.031.6623028
AGAGTAC104650.031.02381511
CAGAGTA108350.030.02836810
GCAGAGT109200.030.01449
CGAATTA2050.028.43017415
AGACTCG5950.028.25481625
GTGGTAT8800.028.0545961
ACTCGAC6250.025.81549827
AGTACGG60800.025.43006513
GAGTACG61050.025.38266612
GAGTACT67200.025.35546512
GACTCGA6700.025.09484526
Produced by FastQC (version 0.11.5)