FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443633
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29800.038.409991
GGTATCA13450.034.2451251
TCAACGC35850.031.2557284
ATCAACG35850.031.1598513
CAACGCA36350.030.82585
AACGCAG37850.029.6051966
TATCAAC38250.029.2047232
GTGGTAT6650.027.911831
ACGCAGA42150.026.6637527
CGCAGAG42550.026.3323218
TGGTATC7000.026.0242982
AGAGTAC42850.025.74692211
TCGTATG1101.1228476E-625.16760640
CAGAGTA45000.024.66954210
GCAGAGT47100.024.0074529
AGTACGG36300.020.6398213
GAGTACG36350.020.61214412
TATACGA850.004210525720.2186555
GTAGTTT6550.019.65395470
GAGTACT18900.018.73037712
Produced by FastQC (version 0.11.5)