FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594960
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51920.3255254050258313No Hit
CCCCTATACTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA50630.3174374278978783No Hit
GCCTTGGGCTGACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGAGTCC44990.28207603952450216No Hit
GTATAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTT44910.2815744595475749No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43800.2746150373677083No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42320.26533580779455285No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40900.2564327632040929No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT38970.24433214626072125No Hit
AGCCAGGACAGGCCCCTATACTTGTCATCTATGGTAAAAACAACCGGCCC38810.24332898630686664No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC35720.22395545969804884No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33080.20740332045944726No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32680.20489542057481064No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31590.19806139338917592No Hit
GACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGAGTCCTCGAGGGTTT31120.19511461102472788No Hit
GTCTATGGTGAGTCCTTCGGTGATTACTACTGGAGCTGGATCCGCCAGCC30090.18865676882178864No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29830.18702663389677485No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29770.18665044891407936No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29560.18533380147464515No Hit
TCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG28970.18163464914480615No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25820.16188493755329286No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGTTCACATGCCAAGGAGACAGCCTCAGAAC24870.15592867532728094No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24710.1549255153734263No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24370.15279380047148516No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGGTTCTGAGGCTGTCTCCTTGGCATGTGAACCTGACTGT24350.15266840547725335No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24320.1524803129859056No Hit
CTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA24030.15066208556954408No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23840.14947083312434167No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGA23810.14928274063299393No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23620.14809148818779153No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22400.14044239353964988No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22270.139627326077143No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCAAGGACGGTGACCTTGGTC22220.13931383859156343No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC21820.1368059387069268No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21220.1330440888799719No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21180.13279329889150823No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21040.13191553393188543No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21010.1317274414405377No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20790.13034809650398757No Hit
GTTCCGAAGAGTCCTCGAGGGTTTCCACTGCTGTCCCGGGAGCTACAGTA20660.12953302904148067No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19840.12439183427797564No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCGTGGTGGCAGCTCCCAGATGGGTC19800.12414104428951195No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG19740.12376485930681647No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19430.1218212368962231No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA18820.11799668957215227No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18480.11586497467021117No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18280.11461102472789285No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGTTCACATGCCAAGGAGACAGCCTCAGAACCTATTATGCAAGCTGGTA18260.11448562973366103No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18230.11429753724231327No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17760.11135075487786529No Hit
GACTATTACTGTAGCTCCCGGGACAGCAGTGGAAACCCTCGAGGACTCTTCGGAACTGGGACCAAGGTCACCGTC17620.11047298991824248No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG17370.10890555249034459No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC17220.10796509003360587No Hit
GAAATAAACAGCCGTGTCCGCGGCGGTCACGGAGCTCAGCCTCAGGGAGA17130.10740081255956262No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17110.10727541756533078No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG16550.10376435772683953No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16470.10326277774991223No Hit
CTATTACTGTAGCTCCCGGGACAGCAGTGGAAACCCTCGAGGACTCTTCG16460.10320008025279631No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAGCTCCCGGGACAGCAGTGGAAACCCTCGAGGA16320.1023223152931735No Hit
CTCTTTGCATAGATTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT16130.1011310628479711No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA16030.10050408787681195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)