FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443911
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCT74660.5170678802225345No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73010.5056405831107319No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTTGTTGGTACTGTATAAAACATT61700.42731165563528495No Hit
GTTTTATACAGTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAA60840.42135560986792125No Hit
GTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG55950.3874892566093063No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50650.35078339315927365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49870.34538139816096697No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47650.33000648931963256No Hit
ATATTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT45240.3133157099017876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44570.3086755347109344No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43500.30126510567479575No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43180.29904890259856737No Hit
CTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAATCTGTC40250.27875679318185126No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTGGGCCGAACGTC38550.266983214339388No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38520.2667754453009915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36140.25029243492154296No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC35890.24856102626823953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35370.2449596962693684No Hit
CACCATCACCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACTCCTCTGACGTT34210.2369259601180405No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33390.2312469397352053No Hit
GCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC30610.2119936755104712No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30330.21005449781877136No Hit
TTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT30200.20915416531905362No Hit
GTACTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAAT29080.20139745455225425No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28880.20001232762961152No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28750.19911199512989375No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28490.19731133013045818No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCGGCC27730.19204784782441578No Hit
CCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG27340.18934685032526244No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAA26680.1847759314805414No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA26520.1836678299424272No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGTACCAACAAGAGGAACTA25520.1767421953292135No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAATCTGGAGA25510.17667293898308137No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24940.17272532725354955No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24290.16822366475496064No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24070.16670002514005366No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23830.16503787283288235No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23220.16081323571882203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22910.15866628898872576No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22850.15825075091193294No Hit
CGTCAGAGGAGTACTATAATATTGCTGACAATAATAAACTGCCACATCTT22630.15672711129702593No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22400.15513421533598679No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21760.15070180918353002No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21370.14800081168437668No Hit
TGTTGGTACTGTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCC21200.14682345380013034No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21150.14647717206946964No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21120.14626940303107325No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20770.14384543091644844No Hit
CTTCGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG20090.13913599937946314No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC20040.13878971764880246No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19910.13788938514908466No Hit
GCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT19860.137543103418424No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19760.1368505399571026No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA19240.1332492099582315No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT18930.13110226322813526No Hit
GTCCCTGGGCCGAACGTCAGAGGAGTACTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGG18760.12992490534388892No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18690.12944011092096397No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACTCCTC18610.12888606015190687No Hit
TCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC18450.12777795861379268No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTTGTTGGTACTG18440.12770870226766054No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18420.12757018957539626No Hit
GATTTCACTCTCACCATCACCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACT17720.12272224534614667No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17650.12223745092322172No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17580.12175265650029676No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17540.1214756311157682No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT17380.12036752957765402No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17140.11870537727048273No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16460.11399594573349742No Hit
GGATCTCTGGTGCCTTCGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGG16360.11330338227217605No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16350.11323412592604391No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16260.11261081881085468No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16240.11247230611859042No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16180.1120567680417976No Hit
CCACTGAATCGGTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAAT16120.11164122996500477No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16110.11157197361887264No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCGGCCTGCAGGCTGAAGATG16080.11136420458047623No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16060.11122569188821195No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15600.10803989996613365No Hit
TTCGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCT15410.10672402938962304No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15280.10582369688990527No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15270.10575444054377313No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15200.10526964612084816No Hit
GTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATA15180.10513113342858389No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14960.10360749381367688No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14950.10353823746754474No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14760.10222236689103414No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14580.10097575266065567No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14510.10049095823773072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1650.047.8327331
GGTATCA10650.044.4642331
CTACGCT1550.044.3278128
GTGGTAT3250.043.2894251
TCGTATG404.941304E-543.2152640
TGGTATC3550.040.5991822
GTATCAA26600.039.603911
TACGCTG1850.037.14852529
ACTACGC1850.037.12663727
CGAGACT1850.037.10220723
ACGAGAC2000.036.0342722
CGTATGC501.8306344E-434.59876641
CGCCTAA400.002661930634.32548524
TATCAAC30900.034.204922
ATCAACG31650.033.50283
TCAACGC31950.032.9745524
AACGCAG32150.032.6626856
CAACGCA32450.032.3607145
CACGAGA2150.031.9240521
ACATCTC2600.031.6740918
Produced by FastQC (version 0.11.5)