FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443911
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73830.5113196034935671No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCT70980.4915815448459081No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTTGTTGGTACTGTATAAAACATT55630.3852730535330779No Hit
GTTTTATACAGTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAA53250.3687900431536293No Hit
GTACCAACAAGAGGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG48170.3336078193185037No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46390.32128018970698335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46210.3200335754766049No Hit
ATATTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT45640.31608596374707304No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45050.311999839325277No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44730.3097836362490486No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTGGGCCGAACGTC42400.2936469076002607No Hit
CTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAATCTGTC42220.2924002933698822No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42190.2921925243314858No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40380.27965712568156903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39220.2716233895302411No Hit
CACCATCACCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACTCCTCTGACGTT36430.2523008689593749No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC35840.24821474453757883No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35550.24620631049974687No Hit
GTACTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAAT33390.2312469397352053No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32810.22723007165954134No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTC32370.22418279242972733No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29590.20492952820499324No Hit
GCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC29530.20451399012820043No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC29480.20416770839753973No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29290.2028518378210291No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCGGCC27360.1894853630175267No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTGCCGGGT26980.1868536218645055No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26970.18678436551837335No Hit
TTATAGTACTCCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT26630.18442964974988071No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26370.18262898475044514No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25720.17812732225185623No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25560.17701922071374207No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAA25480.17646516994468495No Hit
CCTCTGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG25470.17639591359855283No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24440.16926250994694272No Hit
CGTCAGAGGAGTACTATAATATTGCTGACAATAATAAACTGCCACATCTT23860.16524564187127877No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23800.16483010379448595No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA23710.16420679667929672No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23340.16164431187240766No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23290.16129803014174698No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22640.15679636764315805No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21970.15215619245230488No Hit
TCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21900.15167139802937993No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21800.15097883456805855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21680.15014775841447292No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGTACCAACAAGAGGAACTA21570.1493859386070194No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21200.14682345380013034No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21100.14613089033880897No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20610.14273732937833425No Hit
GTCCCTGGGCCGAACGTCAGAGGAGTACTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGG20510.1420447659170129No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20370.14107517707116296No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAATCTGGAGA20230.14010558822531305No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACTCCTC20090.13913599937946314No Hit
GCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT19810.13719682168776332No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTCTTGTTGGTACTG19030.1317948266894566No Hit
GATTTCACTCTCACCATCACCGGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGTACT18760.12992490534388892No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18670.1293015982286997No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18580.12867829111351045No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18530.12833200938284978No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18510.1281934966905855No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18440.12770870226766054No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18100.1253539864991679No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT18020.12479993573011078No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17710.12265298900001455No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCGGCCTGCAGGCTGAAGATG17250.11946719707793625No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17210.1191901716934077No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG17120.11856686457821847No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17010.11780504477076495No Hit
TGTTGGTACTGTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCC17000.11773578842463282No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16910.11711248130944359No Hit
CTTCGGGGACATCGTGATGACCCAGTCTCCAGATTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16770.11614289246359367No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16750.1160043797713294No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16720.115796610732933No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT16160.11191825534953331No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15340.10623923496669808No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCCGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14770.10229162323716627No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14730.10201459785263774No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14690.10173757246810919No Hit
CCACTGAATCGGTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAAT14660.10152980342971278No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14630.10132203439131636No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14480.10028318919933432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)