FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106653
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGAGTGGCTTGCGCTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCAT66010.596483269823513No Hit
CTCTTTATACTGCTGACAGTAATAGACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT50760.458680363221353No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44840.4051857266912031No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCTA35480.3206063689340742No Hit
ACTATACCCCGTCCCTCAAGAGTCGGGTCACCATGGCCGTAGACACGTCG34920.31554606547851943No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATATCAGTGGCCCTGGT28430.2569007629311085No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27680.2501235708031334No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27640.24976212055630806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27640.24976212055630806No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26690.24117767719420632No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT26550.23991260133031764No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26380.23837643778130993No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25430.2297919944192082No Hit
CTATTACTGTCAGCAGTATAAAGAGTGGCTTGCGCTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAA25390.22943054417238284No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25300.22861728111702584No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25080.2266293047594865No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAACAGATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCGCCCAGGCT24640.22265335204440778No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATAAAGAGTGG23580.21307492050353632No Hit
CTGATATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT23510.212442382571592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23300.21054476877575898No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTGAGCGCAAGCCACTCTTTATACTG22930.20720135399262463No Hit
GTCTATTACTGTCAGCAGTATAAAGAGTGGCTTGCGCTCACTTTCGGCGG22710.20521337763508524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22360.20205068797536355No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21810.19708074708151518No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21580.1950024081622695No Hit
CAGTAATAGACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA21040.1901228298301274No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20720.1872312278555247No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAACAGATTAGCCTGGTA20700.18705050273211205No Hit
GTAATAGACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA20440.18470107612774736No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20410.18442998844262834No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20150.18208056183826368No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19570.17683953325929627No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19470.17593590764223294No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC18420.16644783866306784No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGGTTCAGGGAGAACTGGTTCTTCGACGTGTCTACGGC18290.16527312536088548No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCGGGCTGGGATATCA17670.15967064653509275No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAA17350.15677904456049005No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17340.15668868199878372No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17300.1563272317519584No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17040.15397780514759368No Hit
GTGTATTATTGTGCGAGACTAGGGGACGTATTATTATGGTTCGGGGAGTC16990.15352599233906203No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16980.1534356297773557No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16970.15334526721564934No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16910.15280309184541135No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGGTTCAGGGA16760.1514476534198163No Hit
GTTAGCAACAGATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCGCCCAG16520.1492789519388643No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16460.1487367765686263No Hit
GGGTATAGTAGGTGCTCCCAGCATAATGGATACTCCCGATCCACTCCAGC16350.14774278838985663No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15880.1434957479896589No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15760.1424113972491829No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15670.1415981341938259No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15520.14024269576823087No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15180.1371703686702155No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14950.13509202975096982No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14560.13156788984442278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTGAGCGCAAGCCA14410.13021245141882776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14340.1295799134868834No Hit
GTATTATTGTGCGAGACTAGGGGACGTATTATTATGGTTCGGGGAGTCCT14260.12885701299323274No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14200.12831483762299473No Hit
CTGCTACTCTGGCTCCCAGATTCTACTGGAGAAATATTGATGATGCAGTC14190.1282244750612884No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGCAAGCCACTCTTTATACTGCTG14100.12741121200593142No Hit
GACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14060.12704976175910604No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13740.12415815978450336No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13560.12253163367378933No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13550.122441271112083No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13520.12217018342696401No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13520.12217018342696401No Hit
GTCTCTGGTGGCTCCATCAGCAGGAGTAGTTATTACTGGGGCTGGATCCG13360.12072438243966267No Hit
TCTATGATGCATCCACCAGGGCCACTGATATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT13190.11918821889065496No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13170.11900749376724232No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13070.11810386815017895No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12980.11729060509482196No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12940.11692915484799661No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12900.11656770460117129No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12880.11638697947775861No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12510.11304356469462425No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG12390.11195921395414823No Hit
CATCTATGATGCATCCACCAGGGCCACTGATATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA12300.11114595089879122No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC12250.11069413809025955No Hit
GTATAGTAGGTGCTCCCAGCATAATGGATACTCCCGATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAG11990.10834471148589485No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11870.10726036074541884No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA11820.10680854793688717No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11810.10671818537518084No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11750.10617601000494283No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11740.1060856474432365No Hit
GCCGTAGACACGTCGAAGAACCAGTTCTCCCTGAACCTGAGTTCTGTGAC11440.10337477059204647No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA11390.1029229577835148No Hit
GGATATCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGCGCCTGGCCAGGTTTCTGCTGGTACCAGG11390.1029229577835148No Hit
TCATAGATGAGGAGCCTGGGCGCCTGGCCAGGTTTCTGCTGGTACCAGGC11340.10247114497498314No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11230.10147715679621344No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC11150.10075425630256278No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATT11090.10021208093232478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)