FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106653
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGAGTGGCTTGCGCTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCAT60760.5490429249276874No Hit
CTCTTTATACTGCTGACAGTAATAGACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT50350.4549754981913933No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46060.4162099592193759No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCTA34970.3159978782870511No Hit
ACTATACCCCGTCCCTCAAGAGTCGGGTCACCATGGCCGTAGACACGTCG30670.27714197675332736No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29720.2685575333912256No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29060.26259360431860757No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28630.25870801416523515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27430.24786450676047506No Hit
GTCCCAGACCCACTGCCACTGAACCGGGCTGGGATATCAGTGGCCCTGGT26510.2395511510834923No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26440.23891861315154797No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT26020.2351233855598819No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25960.23458121018964392No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24220.21885812445274172No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAACAGATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCGCCCAGGCT23780.21488217173766302No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTGAGCGCAAGCCACTCTTTATACTG23600.213255645626949No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATAAAGAGTGG23360.21108694414599696No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23140.20909896778845763No Hit
GTCTATTACTGTCAGCAGTATAAAGAGTGGCTTGCGCTCACTTTCGGCGG22360.20205068797536355No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22340.20186996285195086No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22310.2015988751668319No Hit
CTATTACTGTCAGCAGTATAAAGAGTGGCTTGCGCTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAA22290.20141815004341923No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21630.19545422097080115No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21290.19238189387278576No Hit
CTGATATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT21290.19238189387278576No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20150.18208056183826368No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAACAGATTAGCCTGGTA19750.17846605937001028No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19470.17593590764223294No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19190.17340575591445556No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19150.1730443056676302No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19090.17250213029739223No Hit
GTAATAGACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA18490.16708037659501218No Hit
CAGTAATAGACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA18380.1660863884162425No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18040.16301406131822713No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGGTTCAGGGAGAACTGGTTCTTCGACGTGTCTACGGC17830.1611164475223941No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17650.15948992141168009No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAA17620.1592188337265611No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17510.15822484554779143No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC17420.1574115824924344No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCGGGCTGGGATATCA17090.15442961795612536No Hit
GTGTATTATTGTGCGAGACTAGGGGACGTATTATTATGGTTCGGGGAGTC16990.15352599233906203No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16920.15289345440711768No Hit
GGGTATAGTAGGTGCTCCCAGCATAATGGATACTCCCGATCCACTCCAGC16830.15208019135176065No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16630.15027294011763398No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16410.14828496376009462No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16180.14620662484084895No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15860.14331502286624626No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGGTTCAGGGA15830.14304393518112724No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15510.14015233320652454No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14910.1347305795041445No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14870.13436912925731914No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14840.13409804157220015No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14630.13220042777636712No Hit
GTTAGCAACAGATTAGCCTGGTACCAGCAGAAACCTGGCCAGGCGCCCAG14620.13211006521466076No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14480.1308449893507721No Hit
CTGCTACTCTGGCTCCCAGATTCTACTGGAGAAATATTGATGATGCAGTC14400.13012208885712143No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14400.13012208885712143No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14310.12930882580176442No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14210.12840520018470108No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14040.1268690366356934No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13990.12641722382716172No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13870.1253328730866857No Hit
GTATTATTGTGCGAGACTAGGGGACGTATTATTATGGTTCGGGGAGTCCT13780.1245196100313287No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13730.12406779722279704No Hit
GACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13520.12217018342696401No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGCAAGCCACTCTTTATACTGCTG13440.12144728293331333No Hit
GTCTCTGGTGGCTCCATCAGCAGGAGTAGTTATTACTGGGGCTGGATCCG13380.12090510756307532No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13240.11964003169918663No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTGAGCGCAAGCCA13120.11855568095871062No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13110.11846531839700429No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13110.11846531839700429No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13100.11837495583529797No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13010.11756169277994095No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12880.11638697947775861No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12800.11566407898410792No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12670.11448936568192558No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12620.11403755287339391No Hit
TCTATGATGCATCCACCAGGGCCACTGATATCCCAGCCCGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT12580.11367610262656858No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12520.11313392725633058No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA12130.10960978734978354No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12130.10960978734978354No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11990.10834471148589485No Hit
GGATATCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGCGCCTGGCCAGGTTTCTGCTGGTACCAGG11910.10762181099224419No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11810.10671818537518084No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11620.10500129670276048No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11600.10482057157934782No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11570.10454948389422881No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATT11570.10454948389422881No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11450.1034651331537528No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC11280.10192896960474512No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11110.10039280605573743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA350.003768585261.28022870
GAACCGT200.007529066451.451747
GTGGTAT2700.041.9881441
GGTATCA8250.041.6411361
TGGTATC2800.039.231462
GTATCAA19450.038.1514171
GTAGTTT750.001588178638.1299270
GAGGGTT1157.854676E-637.30100370
ACCTGAG13350.036.41621470
ATTAGGG501.822214E-434.62502343
TATAAGA1253.6379788E-1232.954432
TATCAAC23100.032.6928862
TGTTAGA7350.032.09916370
TCAACGC23400.031.807764
AACGCAC553.05037E-431.7159954
ATCAACG23750.031.632143
AACGCAG23800.031.418716
ACGAACT18000.031.03543955
CAACGCA24100.030.8852775
AACGAAC18350.030.60981854
Produced by FastQC (version 0.11.5)