FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286176
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT142250.6222180619514858No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG93190.40762390997018605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC91330.3994880534132105No Hit
CTACAGACCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG90610.3963386895847039No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG89730.39248946712764027No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC87510.3827789286564114No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT84770.3707938496423723No Hit
GGTCTAGTCAGAGCCTCCTGAAGATTAATGGGAACGACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTC77070.3371131531430651No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG74690.32670275604327925No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC72670.3178670408577467No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT72190.31576746497207564No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT71110.31104341922931567No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC64500.28213050963705333No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAATCTGTGCCTGATCCACTGCCA62500.2733822767800904No Hit
CTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC61850.2705391011015775No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG59910.2620533152303235No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCC59900.26200957406603864No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT59300.2593851042089498No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGGA58310.2550547289447532No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC54800.2397015802807833No Hit
GCTCTACAGACCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT52240.22850384222387077No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG51810.22662297215962374No Hit
GTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGA49920.21835589210979384No Hit
GTCAGGGACCCCGGGGGCCCGATCAGAACCCAAGTAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATA49410.2161250927312683No Hit
GTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGC49360.21590638690984423No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGGAAATCAGCAGAG49330.2157751634169898No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT48910.2139380345170276No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA48850.21367558753131866No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG48250.21105111767422982No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG48050.2101762943885335No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC47590.20816420083143206No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG47040.20575843679576725No Hit
GGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAAT46230.20221540248869727No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA45710.19994086194588695No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTCCACTCTCC45510.19906603866019063No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTCCACT44330.19390458127458254No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA44040.19263608751032293No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42820.18729966546757554No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAATCTGTGCCTGATCCACTGCCA42080.18406281931049928No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA41990.18366914883193594No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41970.18358166650336633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41650.18218194924625225No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT41230.18034482034629004No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG41220.18030107918200522No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGA40790.17842020911775822No Hit
GATTTTACACTGGAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAGACC40650.17780783281777082No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA40650.17780783281777082No Hit
CTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCCCT39710.17369616337499827No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39490.17273385776073233No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA38730.16940952927508643No Hit
AGTCAGAGCCTCCTGAAGATTAATGGGAACGACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAA38620.16892837646795347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG38510.16844722366082052No Hit
GCCCTGGCTTCTGCACATACCAATCCAAATAGTCGTTCCCATTAATCTTC38160.166916282910852No Hit
TTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGA37730.16503541284660497No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC37010.16188604901809833No Hit
GTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCCCTGA36960.16166734319667425No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36830.16109870806097168No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGACCGGGGT36720.16061755525383872No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC36620.16018014361099056No Hit
GGGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAAT35800.1565933681396358No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCGGGGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT35560.15554358019680023No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG35450.1550624273896673No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34760.1520442870540151No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT34250.14981348767548955No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG33940.1484575115826603No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGACA33050.1445645479613118No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC32960.14417087748274848No Hit
GTTTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA32190.14080280783281776No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC32140.14058410201139368No Hit
GGTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCC31730.13879071427571632No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG31690.13861574961857706No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGA31450.1375659616757415No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTC31300.13690984421146926No Hit
GGCCGAAGGTGACCGGGGTCTGTAGAGCTTGCATGCAGTAATAAACCCCA31220.13655991489719077No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA31210.13651617373290595No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT30830.134854009490083No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT30310.13257946894727266No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCACATACCAATCCAAATAGTCGTTCCCATTAATCTTCAGGAGGCTCTGA30070.1315296810044371No Hit
CTTCTGCACATACCAATCCAAATAGTCGTTCCCATTAATCTTCAGGAGGC29630.12960506977590527No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA29000.12684937642596197No Hit
GTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCC28950.1266306706045379No Hit
GCACATACCAATCCAAATAGTCGTTCCCATTAATCTTCAGGAGGCTCTGA28840.1261495177974049No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT28450.12444361239029716No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC28300.12378749492602495No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT27920.12212533068320199No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGAAGATTAATGGGAACGACTATTTGG27420.11993827246896127No Hit
GGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGT27260.11923841384040425No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC27240.1191509315118346No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC27180.11888848452612573No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC26980.11801366124042945No Hit
CTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCCCTGACAGGTT26970.11796992007614462No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA26770.11709509679044834No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT26590.11630775583332167No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT26240.11477681508335316No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT25990.11368328597623278No Hit
GGTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGT25790.11280846269053652No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA25660.11223982755483393No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG25310.11070888680486542No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT25200.11022773399773245No Hit
GTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAG24930.10904672256204245No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24810.1085218285906247No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAACACAATGTCC24600.1076032641406436No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC24500.10716585249779544No Hit
GCCTCCACTCTGCTGATTTCCAGTGTAAAATCTGTGCCTGATCCACTGCC24470.10703462900494101No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24360.10655347619780804No Hit
GCAGTGGATCAGGCACAGATTTTACACTGGAAATCAGCAGAGTGGAGGCT24170.10572239407639655No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23700.10366655935501029No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC23400.10235432442646585No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG23400.10235432442646585No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA23390.10231058326218104No Hit
GAACCCAAGTAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATA23360.1021793597693266No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23110.10108583066220622No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22960.100429713197934No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATACCAATCCAAAT22870.1000360427193707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)