FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286176
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT143050.625717355094271No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT87610.38321634029925955No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC82350.3602084878854472No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG82180.3594648880926053No Hit
CTACAGACCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG81200.3551782539926935No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC81080.3546533600212757No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG76950.3365882591716473No Hit
GGTCTAGTCAGAGCCTCCTGAAGATTAATGGGAACGACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTC70960.3103873017650435No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT70470.30824398471508757No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC69740.30505087972229605No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT64660.28283036826561037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC62930.27526314684433745No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAATCTGTGCCTGATCCACTGCCA62410.2729886063015271No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC59960.2622720210517476No Hit
CTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTC58830.2573292694875635No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG57380.2509868006662654No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT55390.24228230897358735No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCC52580.2299910418095545No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGGA52110.22793520708816817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT51650.22592311353106673No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA51400.22482958442394635No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT49850.2180497039598001No Hit
GCTCTACAGACCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT49750.217612292316952No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG49670.21726236300267346No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGGAAATCAGCAGAG48800.2134568817098946No Hit
GGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAAT48650.2128007642456224No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA48230.21096363534566018No Hit
GTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGC47720.20873283596713463No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA45640.19963467379589322No Hit
GTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGA45500.19902229749590583No Hit
GTCAGGGACCCCGGGGGCCCGATCAGAACCCAAGTAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCACATA43730.19128011141749365No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTCCACTCTCC43510.19031780580322774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG43010.18813074758898704No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC42990.1880432652604174No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42920.18773707711042367No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG42630.18646858334616406No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTCCACT41860.18310051369623334No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41280.18056352616771412No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAATCTGTGCCTGATCCACTGCCA41220.18030107918200522No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA40940.17907632658203043No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40850.1786826561034671No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA40130.17553329227496048No Hit
CTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCCCT39260.1717278109821816No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG39210.1715091051607575No Hit
GATTTTACACTGGAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAGACC38870.17002190557507385No Hit
GGGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTCCAGTGTAAAAT38820.16980319975364977No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT38310.16757240037512422No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA38280.16744117688226978No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA38050.16643513010371905No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37770.16521037750374423No Hit
TTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGA37340.16332950743949723No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT37240.16289209579664907No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC36950.16162360203238946No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGA36570.1599614377895665No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36520.15974273196814243No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG36470.15952402614671835No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC36250.15856172053245243No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG35770.15646214464678135No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGACCGGGGT35250.154187604103971No Hit
AGTCAGAGCCTCCTGAAGATTAATGGGAACGACTATTTGGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAA35140.15370645129683802No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT34940.15283162801114175No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCGGGGTCTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT34130.14928859370407177No Hit
GTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCCCTGA33910.14832628808980586No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG33690.14736398247553995No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGACA33080.14469577145416626No Hit
GGCCGAAGGTGACCGGGGTCTGTAGAGCTTGCATGCAGTAATAAACCCCA33010.14438958330417256No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC32340.14145892529708998No Hit
GTTTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA32060.1402341726971152No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31970.13984050221855185No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGTTGACTCAGTCTC31820.13918438475427963No Hit
GCCCTGGCTTCTGCACATACCAATCCAAATAGTCGTTCCCATTAATCTTC31500.13778466749716556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31060.13586005626863373No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAGACCCCGGTCACCTTCGGCCAAGGGA30800.13472278599722856No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC30640.13402292736867152No Hit
GGTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGT30160.13192335148300044No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCACATACCAATCCAAATAGTCGTTCCCATTAATCTTCAGGAGGCTCTGA29860.130611116554456No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA29750.13012996374732302No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT29370.1284677995045001No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC28860.12623700012597455No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG28350.12400620074744902No Hit
CTTCTGCACATACCAATCCAAATAGTCGTTCCCATTAATCTTCAGGAGGC28300.12378749492602495No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA28080.12282518931175902No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA28050.12269396581890459No Hit
CTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCCCTGACAGGTT27910.12208158951891718No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT27240.1191509315118346No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC26920.11775121425472056No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC26910.11770747309043574No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT26200.11460185042621392No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA25930.11342083899052392No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT25660.11223982755483393No Hit
GTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGTCC25350.11088385146200466No Hit
GTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAG25240.11040269865487172No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25180.11014025166916283No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT25100.10979032235488431No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG24820.10856556975490951No Hit
GCCTCCACTCTGCTGATTTCCAGTGTAAAATCTGTGCCTGATCCACTGCC24580.10751578181207395No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24370.10659721736209285No Hit
GGATTGGTATGTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACTTGGGTTCTGATCGGGCCCCCGGGGT24130.10554742941925731No Hit
GCACATACCAATCCAAATAGTCGTTCCCATTAATCTTCAGGAGGCTCTGA23970.10484757079070028No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT23570.1030979242193077No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23180.10139201881219993No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23130.10117331299077587No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC23080.1009546071693518No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGAAGATTAATGGGAACGACTATTTGG23000.10060467785507328No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC22930.10029848970507958No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG22910.10021100737650994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)