FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196411
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC53360.4460005800682207No Hit
GTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT47190.39442967341490504No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT40040.334667601685374No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33460.27966977903078455No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT33110.2767443629321362No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30150.2520037010692814No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT29580.24723945199433975No Hit
CTCCTGTGCAGCGTCTGGATTCAGCTTCAGTAGCTATGGCATGAACTGGGTCCGCCAGGCTCCAGGCAAGGGG28320.2367079540392056No Hit
GATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA26610.22241520681438065No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT24860.20778812632113883No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG23790.1988447113909852No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22170.18530421402009845No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC22030.1841340475806391No Hit
CTTTTATACGGTCTGATGAAAGTAAAGAATACTATGCCGACTCCGTGAAG21720.18154296475040768No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21620.1807071315793653No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA21170.17694588230967453No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21160.17686229899257028No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21150.17677871567546602No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20360.17017563362423113No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC19810.16557855118349796No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19620.16399046815851742No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19610.1639068848414132No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT18640.15579930308230197No Hit
GAAATATACAGCCGTGTCCTCAGGTCTCAGGCTGCTCATTTGCAGATACA18610.15554855313098928No Hit
GTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG18340.1532918035691748No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17980.15028280415342218No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG17740.14827680454292044No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC17690.14785888795739924No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17530.14652155488373142No Hit
GTATATTTCTGTGCGAAACGAACGGAGAGCTCCTCTCATCATCGGGCCTT17090.14284388893114489No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16830.14067072268643468No Hit
GTCTCCATCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTT16300.13624080687991No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT16270.13599005692859728No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA15990.13364972404967856No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG15860.13256314092732346No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA15170.12679589204713096No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT15100.1262108088274013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14650.12244955955771052No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT14560.12169730970377236No Hit
GGGCTGGAGTGGGTGTCTTTTATACGGTCTGATGAAAGTAAAGAATACTA14530.12144655975245965No Hit
GTCCCTGATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGT14500.12119580980114693No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGCTCATCACTGAACACAGA14190.11860472697091551No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14140.11818681038539432No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13910.1162643940919968No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATG13770.11509422765253748No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGGCCCGATGATGAGAGGAGCTCTCCGTTCGTTTCGCACAGAAATATACAG13700.11450914443280778No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13520.11300464472493149No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13290.11108222843153398No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13240.11066431184601278No Hit
CAGTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT13170.11007922862628311No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13130.10974489535786616No Hit
GCCTGAGACCTGAGGACACGGCTGTATATTTCTGTGCGAAACGAACGGAG13040.10899264550392801No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT13020.10882547886971951No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC13010.10874189555261526No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGC13000.10865831223551103No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG12790.10690306257632202No Hit
GCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTGTCCACCTTGGTGTTG12630.10556572950265418No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT12630.10556572950265418No Hit
CCCTCAGACTCTCCTGTGCAGCGTCTGGATTCAGCTTCAGTAGCTATGGC12590.10523139623423723No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA12500.10447914638029908No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG12480.1043119797460906No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12420.10381047984346517No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGAC12380.10347614657504822No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAA12260.10247314676979732No Hit
GTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGG12240.10230598013558885No Hit
CGTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG12200.1019716468671719No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA12170.10172089691585919No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA12160.10163731359875494No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC12110.10121939701323375No Hit
GTATAAAAGACACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG12080.10096864706192103No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCGAAGTTG12060.10080148042771256No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG12050.10071789711060831No Hit
GAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCAC12020.10046714715929558No Hit
ATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA12020.10046714715929558No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTTCATGCCATAGCTAC11970.10004923057377439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG501.0950316E-955.31935540
GGACGGT651.7011296E-551.7551970
GTGGTAT2650.045.452711
TGGTATC3000.041.2970352
GGTATCA7300.040.542751
GTATCAA18100.038.5971371
TTAACGG451.0007253E-438.33894335
CTTATAC901.2551027E-1038.2379951
CCTTTGT11750.037.792370
CGTATGC753.929199E-836.89661441
GTGTCTA400.002541624534.65025344
CGAGACA1300.034.42138723
TATCAAC21250.032.8756752
ACGAGAC1056.6029315E-1032.78090322
ATCAACG22300.031.6363683
TCAACGC22250.031.5541084
CAACGCA22750.030.8606115
AACGCAG23400.030.1517076
ATACACA1750.029.4991154
CACGAGA1202.752131E-928.68328921
Produced by FastQC (version 0.11.5)