FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275751
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25760.11319340296895399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79350.042.9132841
GGTATCA29500.041.3496061
TATCAAC90850.037.5955122
ATCAACG92850.036.822713
TCAACGC93600.036.5651664
CAACGCA96100.035.721215
AACGCAG98750.034.798186
GTGGTAT10350.034.1949421
TGGTATC10100.033.6812632
ACGCAGA107000.032.0459827
CGCAGAG109150.031.414758
AGAGTAC109400.030.93332711
CAGAGTA110650.030.553510
GCAGAGT117850.029.0658389
AGTACGG70300.025.5590413
GAGTACT62050.025.41432812
GAGTACG71450.025.196312
AGTACTT63250.024.87729513
GTACTTT64950.024.27905314
TTAACGG6150.024.11852635
Produced by FastQC (version 0.11.5)