FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558930
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14250.25495142504428103No Hit
GTCTCTGGTGGTTCCATCACCAGTAGTGCTTACTACTGGGGCTGGATCCG13690.24493228132324263No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13290.23777575009392948No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12790.22883008605728805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT12150.21737963609038696No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11660.2086128853344784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11470.20521353300055464No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11200.20038287442076824No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10880.19465764943731773No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10830.19376308303365358No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCA10830.19376308303365358No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10540.18857459789240155No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCACCAGTAGTGCTTACTACTGGG10470.18732220492727175No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10220.18284937290895104No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10130.18123915338235558No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9500.16996761669618735No Hit
GTGCAGCCACAGTTCTTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTG9430.16871522373105757No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9090.16263217218614137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9010.16120086594027874No Hit
CACTATCAGCGGCCTGCTACCCGAAGATTTTGCAACTTACTATTGTCAACAGGCTGACAGTTTCCCCGTCACTTT8920.15959064641368328No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8710.15583346751829388No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8700.15565455423756105No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8560.15314976830730145No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8210.14688780348165245No Hit
GACCAAGGTGGAGCTCAAAAGAACTGTGGCTGCACCATCTGTCTTCATCT8150.14581432379725548No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGATT8150.14581432379725548No Hit
ACCTTGGTCCCTCCGCCGAAAGTGACGGGGAAACTGTCAGCCTGTTGACA8030.14366736442846154No Hit
ACGTACTACAATCCGTCCCTCAAGAGTCGAATCACCATTTCCGTGGACAC7770.139015619129408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7420.132753654303759No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7400.1323958277422933No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7350.13150126133862916No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7340.13132234805789633No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7240.12953321525056805No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7220.1291753886891024No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7110.12720734260104127No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGGCGGGAGTTTCCACCGAAGGCGTAGCCAGGCTGTCTCGCACAGAAATAAA7070.12649168947810996No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGACGGGGAAA7050.1261338629166443No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6970.12470255667078167No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6970.12470255667078167No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGGCAT6890.12327125042491903No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6860.12273451058272056No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6750.12076646449465944No Hit
GCATAGATCAGGAGTTGAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCTAACCTGCTGATACCCCGACTCGCC6670.11933515824879681No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6500.11629363247633873No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA6480.11593580591487307No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6470.11575689263414024No Hit
GGGTATCAGCAGGTTAGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTCAACTCCTGATCTATGCTGCGTCCA6420.1148623262304761No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6410.11468341294974325No Hit
GAAATAAACAGCCGAGTCGGCGGCACTCACAGAGTTCACGTTCAGGGAGA6330.11325210670388063No Hit
CTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAATCTTGA6210.11110514733508668No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6190.11074732077362102No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6140.10985275436995688No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA6100.10913710124702555No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG6040.1080636215626286No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC6010.10752688172043011No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG5990.10716905515896445No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT5980.10699014187823162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5910.10573774891310182No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA5820.10412752938650635No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5810.10394861610577354No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC5760.1030540497021094No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5750.10287513642137655No Hit
GATCAGGAGTTGAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCTAACC5720.10233839657917806No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5650.10108600361404826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)