FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558930
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14580.2608555633084644No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14320.25620381800941083No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14040.25119424614889163No Hit
GTCTCTGGTGGTTCCATCACCAGTAGTGCTTACTACTGGGGCTGGATCCG13520.24189075555078454No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12190.21809528921331833No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT11530.20628701268495162No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10540.18857459789240155No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCA10150.1815969799438212No Hit
GTGCAGCCACAGTTCTTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTG10020.17927110729429446No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10010.17909219401356163No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9960.1781976276098975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9750.17444044871450806No Hit
CACCTGCACTGTCTCTGGTGGTTCCATCACCAGTAGTGCTTACTACTGGG9600.17175674950351563No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9350.16728391748519492No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9290.16621043780079794No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9120.16316891202833986No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9060.16209543234394289No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8900.15923281985221763No Hit
ACCTTGGTCCCTCCGCCGAAAGTGACGGGGAAACTGTCAGCCTGTTGACA8690.15547564095682823No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8420.15064498237704185No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8380.14992932925411054No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8250.14760345660458377No Hit
CACTATCAGCGGCCTGCTACCCGAAGATTTTGCAACTTACTATTGTCAACAGGCTGACAGTTTCCCCGTCACTTT8200.14670889020091962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8170.14617215035872114No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8130.14545649723578982No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGATT8050.1440251909899272No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8030.14366736442846154No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7970.14259388474406456No Hit
GGGTTCCCTGGCCCCAGTAGTCAAGGCGGGAGTTTCCACCGAAGGCGTAGCCAGGCTGTCTCGCACAGAAATAAA7840.14026801209453776No Hit
GACCAAGGTGGAGCTCAAAAGAACTGTGGCTGCACCATCTGTCTTCATCT7740.13847887928720948No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7470.13364822070742313No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7400.1323958277422933No Hit
ACGTACTACAATCCGTCCCTCAAGAGTCGAATCACCATTTCCGTGGACAC7340.13132234805789633No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7300.13060669493496502No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7180.12845973556617107No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7010.125418209793713No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6890.12327125042491903No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6830.12219777074052207No Hit
GAAATAAACAGCCGAGTCGGCGGCACTCACAGAGTTCACGTTCAGGGAGA6760.12094537777539227No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6730.12040863793319379No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGACGGGGAAA6660.11915624496806397No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA6660.11915624496806397No Hit
GCATAGATCAGGAGTTGAGGGGCTTTCCCTGGTTTCTGTTGATACCAGGCTAACCTGCTGATACCCCGACTCGCC6630.1186195051258655No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6610.11826167856439983No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6550.11718819888000287No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6500.11629363247633873No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6470.11575689263414024No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6460.1155779793534074No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6430.11504123951120893No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6410.11468341294974325No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6330.11325210670388063No Hit
GGGTATCAGCAGGTTAGCCTGGTATCAACAGAAACCAGGGAAAGCCCCTCAACTCCTGATCTATGCTGCGTCCA6310.11289428014241497No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6220.11128406061581952No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6150.11003166765068972No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGGCAT6140.10985275436995688No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT6110.1093160145277584No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6030.10788470828189577No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6000.10734796843969727No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC5920.10591666219383467No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA5910.10573774891310182No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA5870.10502209579017052No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA5860.10484318250943768No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA5840.10448535594797202No Hit
CTGATAGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAATCTTGA5840.10448535594797202No Hit
GTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGATTTCACT5610.10037035049111696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)