FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477829
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10900.037.1147771
CTGACGA1309.392661E-734.6408370
GGTATCA5600.033.059561
CTCGTAT450.00456036430.74818839
TTAACGG902.0017433E-730.64786135
TATCAAC13550.030.3622022
ATCAACG13850.029.7045383
AACGCAG13800.029.5637286
TCAACGC13950.029.2458384
TAACGGC953.2024946E-729.04704736
CAACGCA14050.029.0376835
TTTAACG1103.705827E-828.1862334
ACGCAGA14600.027.9408727
CGCAGAG14750.027.889148
CGGTACC1155.1642928E-827.238645
TAACCGT906.147264E-627.2324953
AGAGTAC15050.027.1054311
CAGAGTA15300.026.43847710
GCAGAGT16300.025.2371049
CTAACCG1001.4027984E-524.48568552
Produced by FastQC (version 0.11.5)