FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477829
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6180.12933497129726323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.037.7405131
CAATCGT700.00176094637.32901870
AACGCAG14150.031.471676
CAACGCA14400.031.4010625
ATCAACG14500.031.1910293
TCAACGC14800.031.0152994
TATCAAC14750.030.6687892
GTTCTAA450.004746807730.4973851
ACGCAGA15550.028.6382087
CGCAGAG15600.028.5464218
GGTATCA5850.028.151431
GTGGTAT2950.027.912861
AGAGTAC15950.027.7052411
TCGTAAA500.00798688127.41603721
CAGAGTA16450.026.86313410
TGGTATC2950.026.7190192
ACGAACC959.44645E-625.76684652
GCAGAGT17350.025.6670999
CGAACCT1001.3955483E-524.50205653
TACGGGA5350.024.33115215
Produced by FastQC (version 0.11.5)