FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739446
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14480.19582227775929548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG350.001393394939.1608529
GTATCAA23950.036.8201261
TATCAAC26400.033.2533762
TCAACGC26650.032.671824
AACGCAG27050.032.5673756
ATCAACG26750.032.554083
CAACGCA26950.032.3081255
GGTATCA11200.031.4333211
AGAGTAC28450.030.84474611
ACGCAGA28700.030.5760637
CAGAGTA29000.030.377510
CGCAGAG30250.029.0093568
GCAGAGT31300.028.145299
CTATCGC500.00796282627.43493730
CGAAACA500.00809563827.34208721
ACGGGTA907.4669406E-626.56280516
GAGTACG26650.026.13745712
AGTACGG26450.026.07690613
GTGGTAT5400.024.0525381
GTCGTAC902.2638268E-422.7866131
Produced by FastQC (version 0.11.5)