FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475642
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41050.27818400397928494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9300.042.1939351
GTATCAA75750.041.7572821
GGTATCA30600.039.474531
TATCAAC85750.036.866372
ATCAACG90100.034.9575163
CAACGCA91050.034.5143935
TCAACGC91150.034.513924
AACGCAG92850.033.9921266
ACGCAGA98200.032.2096337
CAGAGTA99700.031.1780510
CGCAGAG101200.031.153768
AGAGTAC99900.030.9791611
GCAGAGT103650.030.3844879
TGGTATC13050.030.0519142
GAGTACG70750.026.59266712
AGTACGG70250.026.49083113
TTAACGG1759.094947E-1225.54625735
GAGTACT48000.025.2788912
AGTACTT48900.024.60453213
GTTACGT851.5674306E-424.0592216
Produced by FastQC (version 0.11.5)