FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870747
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.041.5957451
TCGTATG1500.036.9229540
GGTATCA8800.036.6802251
TATCAAC27450.034.7747732
ATCAACG27300.034.714293
CTCGTAT1400.034.59323539
CAACGCA27300.034.5885125
TCAACGC27600.034.336964
AACGCAG27950.033.663216
ACGCAGA31400.030.1815327
CTTATCG850.005141579530.0031170
AGAGTAC31400.029.85347411
CGCAGAG31900.029.7084678
CAGAGTA31700.029.67926410
GCAGAGT33450.028.63989
CGTATGC1950.028.43354641
GTCCGAA900.00679809428.33627170
CCGAACG500.007916965527.46798914
TATACAC2300.026.8724063
TACACAT2650.025.9146885
Produced by FastQC (version 0.11.5)