FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802089
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31920.39796082479625083No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9380.11694462833924914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.045.387231
GTATCAA30550.040.7514081
TATCAAC34900.035.6542742
ATCAACG35800.034.84233
TCAACGC36650.034.120834
CAACGCA37250.033.569145
AACGCAG38100.032.9096536
GTGGTAT3550.030.7455711
GCGAACT450.00485010630.36592328
ACGCAGA41600.030.2227177
CGCAGAG42000.029.8537568
AGAGTAC43400.029.12625311
CAGAGTA44100.028.81845510
TCGCATC605.6176225E-428.5752233
TACGGGT2400.028.39351815
GCAGAGT45100.028.0283689
CGAACTA500.00809717427.34129729
TCTGTCG756.7075525E-527.2577768
TGGTATC3900.026.2241132
AGTACTT29050.026.03798113
Produced by FastQC (version 0.11.5)