FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377436
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10700.043.8299061
CTCGTAT350.001330473239.52563539
TATCAAC12800.036.6439172
TCAACGC12900.036.359854
ATCAACG13000.036.0801623
GGTATCA3850.035.5658341
CAACGCA13300.035.2663235
AACGCAG13600.034.996516
TCGTATG501.8237214E-434.6127340
AGAGTAC14500.032.58384711
CGCAGAG14450.032.4639638
CAGAGTA15050.031.6205610
ACGCAGA15200.031.3126667
GCAGAGT15100.031.2891229
TCTTATA553.4023952E-431.1242282
TATTGCG450.004798305730.42854518
GAGTACG10950.027.82704712
CGTATGC755.9408736E-527.74220841
AGTACGG11200.027.5079513
TACGGGT1156.0592356E-826.7903515
Produced by FastQC (version 0.11.5)