FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377436
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8500.22520374315115674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5100.037.647851
GTATCAA10500.036.245661
ACGGCTC450.00480466730.4203038
TCAACGC12800.029.677624
TATCAAC13000.029.5116622
CAACGCA13100.028.997985
ATCAACG13250.028.9356543
AACGCAG13600.027.9318776
TCGGATC500.00746809327.7943843
TCGCTCA756.500127E-527.37827310
ATAGCGG657.2729395E-427.33568263
AATAGCG657.384772E-427.26441662
ATCGTTG658.476466E-426.62866240
AGAGTAC14250.026.1774711
ACGCAGA14650.025.9299347
CGCAGAG14750.025.7541378
CAGAGTA14700.025.60892910
TAGCGGC700.001104894325.44614664
GCAGAGT14850.025.3502529
GTAGTAC1101.230188E-624.8959353
Produced by FastQC (version 0.11.5)