FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010100
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14540.14394614394614394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.038.883241
TATCAAC32500.033.140572
TCAACGC32550.032.852864
GGTATCA13000.032.7459751
ATCAACG32800.032.6089133
CAACGCA33250.032.161225
AACGCAG33600.031.826216
TTAGCGA703.5888705E-529.87792648
GTGGTAT5100.029.6184331
ACGCAGA36550.029.3512467
CGCAGAG37300.028.7610748
AGAGTAC36000.028.75239611
CAGAGTA36550.028.69482810
TAGCGAG755.7250232E-527.9001149
GCAGAGT39550.027.298189
CGTGCGT1207.621854E-427.12019270
CGAACTA3000.025.19815629
AGTACGG32950.024.4445513
GAGTACG33550.024.00738712
TGGTATC6150.023.4254462
Produced by FastQC (version 0.11.5)