FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655283
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.035.247881
GTATCAA42650.034.9743271
ATCAACG49950.030.0011733
TCAACGC50250.029.7538194
CAACGCA51350.029.2508875
AACGCAG51700.029.1191966
TATCAAC53250.028.0123022
ACGCAGA56450.026.6649257
TCGTATG1651.10958354E-1025.17002140
CGCAGAG59950.025.1653678
AGAGTAC58800.025.13197511
CAGAGTA59550.024.75787410
GTGGTAT8900.024.273351
GCAGAGT64600.023.3008529
GTAGTTT7000.021.99474370
AGTACGG48000.020.92858913
CTCGTAT1507.1803333E-720.7462939
GAGTACG48400.020.61394912
TGGTATC10150.020.6083372
TATACAC4800.020.0036413
Produced by FastQC (version 0.11.5)