FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655283
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22960.13870739927855236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45400.035.9703031
GGTATCA21400.033.5062261
ATCAACG51700.031.1474553
TCAACGC52850.030.3975184
TATCAAC53850.029.991962
CAACGCA53750.029.761085
AACGCAG53550.029.6803346
ACGCAGA57400.027.8089317
AGAGTAC58700.027.25141311
CAGAGTA59250.026.94063610
GTGGTAT9700.026.8803441
CGCAGAG60650.026.3752358
TGGTATC10250.025.7594472
GCAGAGT64300.025.1976749
GTAGTTT7950.024.07330770
AGTACGG51500.022.88028713
GCTATAC1052.4068622E-522.8414653
GAGTACG52400.022.55267512
GTACGGG62650.018.64419414
GAGTACT23550.018.32693912
Produced by FastQC (version 0.11.5)