FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001047
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT128620.42858375760193024No Hit
CTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT104870.3494447104627152No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG96530.32165440927782873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC86400.2878995230664498No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG78750.26240841946160787No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC77980.2598426482490944No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGCTGTAAAAAACACT77520.258309849862398No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT76900.25624390421076376No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTA75220.25064585792891614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC74460.24811340842046128No Hit
ATCGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA73690.24554763720794778No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC73530.2450144899430099No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC69860.2327854245534975No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG69500.2315858432073873No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67090.2235553125292606No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAA61660.20546162722543168No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC60620.20199617000333547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT58160.19379903080491576No Hit
GTTTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA57710.192299554122278No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC56920.18966713950164726No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC54950.18310276380209975No Hit
GAGTACGATAATTTTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT54330.1810368181504655No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC53320.1776713260405452No Hit
GTCTCTGGTGGTTCCATTAGTAGTCACTTCTGGAGCTGGATCCGGCAGCC52100.17360607814539394No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC51340.17107362863693903No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAA50980.16987404729082883No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAACAATAAGAACTT50870.16950750854618404No Hit
GTAATAAAGGGCCGTGTCCGCAGCGGTCACGGAGGTCAGCTTCAGGGAGA50020.1666751637012016No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG49730.16570883428350172No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC49380.16454257464145014No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT48320.16101047401123675No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG48300.1609438306031195No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACT47220.1573450865647889No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG46770.15584560988215113No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTT46090.15357973400616518No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA45500.15161375346670677No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA44170.14718196682691073No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT43940.14641556763356256No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43320.1443496219819283No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG43090.1435832227885801No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42070.14018440897460122No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA42060.1401510872705426No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACTCCGC41750.13911811444472544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41630.13871825399602206No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGCTG41530.13838503695543589No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41180.1372187773133843No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT41020.13668563004844642No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG40370.13451971928463632No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT40350.13445307587651908No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT40240.1340865371318743No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG39860.13282031237764688No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39470.1315207659193608No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG39350.1311209054706574No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38940.1297547156042541No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT38940.1297547156042541No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC38930.1297213939001955No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA38500.12828856062567498No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG38320.12768876995261988No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT37270.12418999102646511No Hit
GTTTTTTACAGCTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA36760.12249058411947564No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC36650.12212404537483085No Hit
CTTCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATC36610.12199075855859637No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG36260.12082449891654479No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA36050.12012474313131383No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG35810.11932502223390704No Hit
CACCTGCACTGTCTCTGGTGGTTCCATTAGTAGTCACTTCTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA34990.11659264250110045No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG34800.11595953012398673No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT33790.11259403801406641No Hit
GTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA33190.1105947357705494No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC33050.11012823191372877No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG33020.11002826680155293No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA32560.10849546841485655No Hit
GTCCTGGACCTCCTGTGCAAGAAGATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC32370.10786235603774282No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA32150.10712927854845326No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC32110.1069959917322188No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC32080.10689602662004294No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG31700.10562980186581548No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT31440.10476343756029147No Hit
ACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT31310.10433025540752944No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC31260.10416364688723635No Hit
TTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTTCGGCGGAGGGA30310.10099808500166774No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT30140.10043161603267127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA350.005124415356.70888570
GTGGTAT6250.046.2074551
TGGTATC6550.044.616722
GGTATCA21150.043.564951
GTATCAA52800.040.7617841
TATCAAC60350.035.7768752
ATCAACG63750.034.0844963
TCAACGC63750.033.9227034
CAACGCA64750.033.506125
AACGCAG65150.033.2487346
TGTTAGA22300.032.33847470
TCGTATG1305.456968E-1231.93437840
CTTATAC3400.031.346971
ACGAGAC3150.030.56405322
CGCAGAG71750.030.3300328
CGTATGC1501.8189894E-1230.00396341
AGAGTAC72300.029.6708611
TATACAC3950.029.5938743
CAGAGTA74150.028.97695210
GCAGAGT75250.028.8731679
Produced by FastQC (version 0.11.5)