FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001047
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT128230.42728421114364423No Hit
CTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT97180.3238203200416388No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG89970.29979537141537604No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG84620.28196825974401596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT82920.27630357005405115No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC77720.25897628394357036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC75890.25287841210084344No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGCTGTAAAAAACACT73340.24438137756589615No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71140.23705060267300046No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC68490.22822035109746697No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC67900.22625437055800857No Hit
GAGTACGATAATTTTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT67480.2248548589875467No Hit
ATCGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA65280.21752408409465096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT64990.2165577546769511No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTA62820.20932694489623122No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC59050.19676466246613267No Hit
GTTTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA58600.19526518578349492No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA56590.1885675232677129No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC55660.18546860479026153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT55160.18380251958733068No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAA53970.1798372368043553No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC53820.17933741124347602No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG51550.17177338442217No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT51340.17107362863693903No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50850.1694408651380668No Hit
GTCTCTGGTGGTTCCATTAGTAGTCACTTCTGGAGCTGGATCCGGCAGCC50790.1692409349137151No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC49520.16500907849827076No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT49380.16454257464145014No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA47300.1576116601972578No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT47020.15667865248361657No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC46600.15527914091315465No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACTCCGCTCACTTT46450.1547793153522754No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACT45900.15294662162905145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT45400.1512805364261206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA44510.14831490476490372No Hit
GTAATAAAGGGCCGTGTCCGCAGCGGTCACGGAGGTCAGCTTCAGGGAGA44500.1482815830608451No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA44440.14808165283649338No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG44160.14714864512285214No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAAAATTATCGTACTCCGC43430.1447161607265731No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG43430.1447161607265731No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43370.1445162305022214No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42820.14268353677899748No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA42550.1417838507694148No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGAAGTTCTTATTGTTGGAGCTG41790.13925140126095992No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC41020.13668563004844642No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAACAATAAGAACTT39930.1330535643060572No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG39570.13185398295994696No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAA39290.13092097524630572No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC38970.12985468071642997No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG38790.12925489004337484No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37850.1261226498618649No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG37830.12605600645374765No Hit
CAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA37620.1253562506685167No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC37510.1249897119238719No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT37450.1247897816995202No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37330.12438992125081678No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT36920.1230237313844135No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT36860.12282380116006181No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36770.12252390582353424No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG36590.12192411515047916No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA35980.11989149120290352No Hit
CACCTGCACTGTCTCTGGTGGTTCCATTAGTAGTCACTTCTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA35310.11765893703097619No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34760.11582624330775226No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG34720.11569295649151778No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT33760.11249407290189056No Hit
GTCCTGGACCTCCTGTGCAAGAAGATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC33710.11232746438159749No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG33610.11199424734101132No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT33570.11186096052477686No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACGATAATTTTGCTGACAGTAATAAACCGCCACATCTTCAGC32880.1095617629447323No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC32620.10869539863920824No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32360.1078290343336842No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG32110.1069959917322188No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC31700.10562980186581548No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT31600.10529658482522934No Hit
CTTCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATC30990.10326396087765369No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC30930.10306403065330198No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA30830.10273081361271584No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30810.1026641702045986No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA30690.10226430975589519No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC30630.10206437953154349No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT30620.10203105782748488No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACGATAATTTTGCTGACAGTAATAAACCGCCACATCTTCAGC30560.10183112760313318No Hit
GTTTTTTACAGCTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCAGAA30520.10169784078689871No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG30240.10076483307325743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6500.047.0689961
GGTATCA23300.042.048181
TGGTATC7800.040.077522
GTATCAA54900.039.5733681
TATCAAC62400.034.7925722
ATCAACG66000.032.9796523
TCAACGC66650.032.6574754
CAACGCA67600.032.1979985
AACGCAG69400.031.3628886
CGCAGAG74750.029.3014098
TGGATTG26350.029.1567970
AGAGTAC75300.028.76824811
CAGAGTA76350.028.55247110
GCAGAGT78500.027.9453959
ACGCAGA79550.027.3180567
TATAAGA4300.027.1621742
GTATAAG5400.026.1004731
TTCGGGG24700.024.82904270
GTATGTA22300.024.5104081
AGTACGG64550.024.41114813
Produced by FastQC (version 0.11.5)