FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310135
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6610.21313299047189127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA350.00563862855.33273770
GTGCATA200.0076069351.3092351
CCTATAC200.0076069351.30923524
GTGGTAT1550.044.136971
GTATCAA13400.043.6511341
TGCATAA350.001361156739.3405335
GTATTAT350.001404106539.0927471
TATCAAC15150.038.3521732
GGTATCA4650.038.252041
TCAACGC15800.036.960894
ATCAACG15850.036.850243
CAACGCA16350.036.1353075
AACGCAG16500.035.806816
TACCCAC400.00222585535.59141563
GTCCTAG400.002704503734.2061541
ACGCAGA18150.032.5516437
TGGTATC2100.032.5510062
CGATATG450.003597151332.27128267
AGAGTAC17600.032.21059811
CAGAGTA17800.032.04054610
Produced by FastQC (version 0.11.5)