FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019699
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.041.248091
GTATCAA24200.040.2712441
TATCAAC27700.035.30672
ATCAACG29650.032.868943
CAACGCA29800.032.8218655
TCAACGC30000.032.7142374
AACGCAG30450.032.2339446
TGGTATC4750.031.064682
ACGCAGA33250.029.4105157
AGAGTAC33600.029.00203711
CGCAGAG34250.028.5518178
GTGGTAT5050.028.5397361
CAGAGTA34650.028.22221210
GCAGAGT35900.027.2395469
AGTACGG27900.024.84013713
GAGTACG28200.024.57588212
CGCGTAT1401.4719262E-824.50636112
GCTATAC750.002055928622.8770923
GCGTATT1606.062692E-821.44306613
ACTAACG850.0042496720.1856672
Produced by FastQC (version 0.11.5)