FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019699
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18400.1804454059482259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG651.8721868E-550.922270
GGTATCA11500.039.4028361
GTATCAA25400.036.4906351
TATCAAC29200.031.4819932
ATCAACG30200.030.3081153
TCAACGC30100.030.2934344
CAACGCA30800.029.6049465
AACGCAG31000.029.4139426
GTGGTAT5800.028.4095671
TGGTATC5700.027.6817322
ACGCAGA33600.027.1378657
AGAGTAC34300.026.68397311
CGCAGAG34350.026.5453368
CAGAGTA35600.025.61326810
TATGTAG1300.001101781825.461170
GCAGAGT36100.025.258519
AGTACGG27400.023.64528713
GAGTACG27850.023.1401412
TACGGGG13850.021.78148715
TATTAGG1507.748713E-720.5807652
Produced by FastQC (version 0.11.5)